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Literature summary extracted from

  • Lee, S.; Park, H.I.; Sang, Q.X.
    Calcium regulates tertiary structure and enzymatic activity of human endometase/matrilysin-2 and its role in promoting human breast cancer cell invasion (2007), Biochem. J., 403, 31-42.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B7 expression in Escherichia coli. Human breast cancer cell line, MDA-MB-231, transfected with wild-type MMP-26 cDNA shows a calcium-dependent invasive potential when compared with controls that were transfected with an inactive form of MMP-26 (E209A) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.B7 D114A mutant enzyme shows high rates of fluorescent synthetic peptide hydrolysis Homo sapiens
3.4.24.B7 D114A mutation induces enhanced affinity for the Ca2+ ion or an irreversible loss of enzymatic activity triggered by low-affinity calcium binding respectively Homo sapiens
3.4.24.B7 D165A very low rates of hydrolysis that are less than 5% of that seen for wild-type MMP-26 Homo sapiens
3.4.24.B7 E191A very low rates of hydrolysis that are less than 5% of that seen for wild-type MMP-26 Homo sapiens
3.4.24.B7 E209A inactive mutant enzyme Homo sapiens
3.4.24.B7 K189E calcium-independent high invasiveness is observed in the K189E mutant MDA-MB-231 cell line Homo sapiens
3.4.24.B7 K189E mutant enzyme shows high rates of fluorescent synthetic peptide hydrolysis Homo sapiens
3.4.24.B7 K189E mutation induces enhanced affinity for the Ca2+ ion or an irreversible loss of enzymatic activity triggered by low-affinity calcium binding respectively Homo sapiens
3.4.24.B7 V184D mutant enzyme shows high rates of fluorescent synthetic peptide hydrolysis Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B7 Ca2+ MMP-26 has one high-affinity and one low-affinity calcium binding site. High-affinity calcium binding is restored at physiologically low calcium conditions with a calcium-dissociation constant of 63 nM without inducing secondary and tertiary structural changes. Low-affinity calcium binding is restored at relatively high calcium concentrations and shows a low-affinity calcium-dissociation constant value of 0.12 mM, which is accompanied with the recovery of enzymatic activity reversibly and tertiary structural changes, but without secondary structural rearrangements Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B7 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B7 endometase/matrilysin-2
-
 Homo sapiens
3.4.24.B7 MMP-26
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.B7 additional information
-
 high-affinity calcium binding protects MMP-26 against thermal denaturation Homo sapiens